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細胞:THP-1

Data

Catalogue No.
(カタログ番号)
88081201
(ご注文時カタログ番号 : 【培養状態】EC86012804-G0)
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=THP1
Cell Line Name
(細胞名)
THP 1
Keywords
(キーワード)
Human monocytic leukaemia
Cell Line Description
(細胞概要)

Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for alpha-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.

Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line.

Please note: We routinely resuscitate and culture this cell line with success, however, some laboratories have found this cell line difficult to establish following resuscitation from a frozen ampoule. Our Quality Control (QC) Department strongly recommend that this line is supplied as a Growing culture whenever possible e.g. to locations within or near the European Union. We routinely resuscitate and maintain this cell line growing in our laboratory to enable us to supply growing cultures quickly. If however, you still wish to receive the cell line Frozen we can supply but our normal replacement Policy will not apply should you encounter resuscitation difficulties.

We recommend, if you are in the European Union (where we can deliver growing cultures), you select a Growing culture.

Species
(種)
Human
Tissue
(臓器)
blood
Morphology
(形態)
Monocyte
Growth Mode
(増殖様式)
Suspension
DNA Profile

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16
Subculture Routine
(継代方法/培養方法)
If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%. To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. Requires 5% DMSO and 95% foetal bovine serum (FBS) as cryoprotectant. Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Replacements will be charged at full cost where claims cannot be substantiated.
Culture Medium
(適応培地)
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype
(核型)
Not specified
Receptors Fc, C3b
Depositor
(供託者)
Dr J Clarke, AVRI, Pirbright
Originator
(創作者)
No
Country
(国名)
UK
References
(文献)
Int J Cancer 1980;26:171; Cancer Res 1982;42:1530; J Immunol 1983;131:1882
Additional Bibliography
(追加文献)
Not specified
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No

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