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細胞:SNL76/7

Data

Catalogue No.
(カタログ番号)
07032801
(ご注文時カタログ番号:【凍結】EC07032801-F0 【培養状態】EC07032801-G0))
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=EC07032801
Cell Line Name
(細胞名)
SNL 76/7
Keywords
(キーワード)
Mouse SIM strain embryonic fibroblast
Cell Line Description
(細胞概要)
SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.
GMO Status Genetically Modified Organism Class 1 (GMO1)
Species
(種)
Mouse
Tissue
(組織)
Embryo
Morphology
(形態)
Fibroblast
Growth Mode
(増殖様式)
Adherent
Subculture Routine
(継代方法/培養方法)
Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO2; 37°C.
Culture Medium
(適応培地)
Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Karyotype
(核型)
Not
Depositor
(供託者)
Profesor Allan Bradley, The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK
Originator
(創作者)
Yes
Country
(国名)
UK
References
(文献)
Thomas and Capecchi (1987) Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51(3): 503-12.
Additional Bibliography
(追加文献)
Williams et al., (1988) Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336(6200): 684-7.
Patents
(特許)
None Specified By Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

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