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細胞:SH-SY5Y

Data

Catalogue No.
(カタログ番号)
94030304
(ご注文時カタログ番号:【凍結】EC94030304-F0 【培養状態】EC94030304-G0))
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=SHSY5Y
Cell Line Name
(細胞名)
SH-SY5Y
Keywords
(キーワード)
Human neuroblastoma
Cell Line Description
(細胞概要)
SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (ECACC catalogue no. 86012802). SH-SY-5Y has dopamine-beta-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
Species
(種)
Human
Tissue
(臓器)
neural
Morphology
(形態)
Neuroblast
Growth Mode
(増殖様式)
Adherent
DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2; using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C. Cells may reattach slowly and may remain in suspension for several days. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
Culture Medium
(適応培地)
Ham's F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Karyotype
(核型)
Not specified
Depositor
(供託者)
Dr PFT Vaughan, Institute for Cardiovascular Research, University of Leeds
Originator
(創作者)
No
Country
(国名)
UK
References
(文献)
Biedler JL, Roffler-Tarlov S, Schachner M, Freedman LS 1978 Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res.38(11 Pt 1):3751-7. PMID: 29704. Ross RA, Spengler BA, Biedler JL. 1983 Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J Natl Cancer Inst.71(4):741-7. PMID: 6137586.
Additional Bibliography
(追加文献)
Jalava AM, Heikkilä J, Akerlind G, Pettit GR, Akerman KE. 1990 Effects of bryostatins 1 and 2 on morphological and functional differentiation of SH-SY5Y human neuroblastoma cells. Cancer Res.; 50(11):3422-8. PMID: 2334938. Påhlman S, Meyerson G, Lindgren E, Schalling M, Johansson I.1991 Insulin-like growth factor I shifts from promoting cell division to potentiating maturation during neuronal differentiation. Proc Natl Acad Sci U S A. 88(22):9994-8. PMID: 1946468. Lopes FM, Schröder R, da Frota ML Jr, Zanotto-Filho A, Müller CB, Pires AS, Meurer RT, Colpo GD, Gelain DP, Kapczinski F, Moreira JC, Fernandes Mda C, Klamt F. 2010 Comparison between proliferative and neuron-like SH-SY5Y cells as an in vitro model for Parkinson disease studies. Brain Res. 14;1337:85-94. PMID: 20380819. Encinas M, Iglesias M, Liu Y, Wang H, Muhaisen A, Ceña V, Gallego C, Comella JX. 2000 Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated, neurotrophic factor-dependent, human neuron-like cells. J Neurochem. 75(3):991-1003. PMID: 10936180. Constantinescu R, Constantinescu AT, Reichmann H, Janetzky B. 2007 Neuronal differentiation and long-term culture of the human neuroblastoma line SH-SY5Y. J Neural Transm Suppl. 2007 ;(72):17-28. PMID: 17982873
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No