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汎用セルライン(株細胞)17品目一覧

細胞:RAW 264.7

Data

Catalogue No.
(カタログ番号)
91062702
(ご注文時カタログ番号:【凍結】EC91062702-F0 【培養状態】EC91062702-G0))
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=RAW264.7
Cell Line Name
(細胞名)
RAW 264.7
Keywords
(キーワード)
Mouse monocyte macrophage
Cell Line Description
(細胞概要)
Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abelson Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.
Species
(種)
Mouse
Tissue
(臓器)
Blood
Morphology
(形態)
Macrophage
Growth Mode
(増殖様式)
Semi-adherent
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Use cell scrapers to remove attached cells. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.
Culture Medium
(適応培地)
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype
(核型)
Not specified
Products(産生物) Lysozyme
Receptors Immunoglobulin, complement
Country
(国名)
USA
References
(文献)
J Immunol 1977;119:950; Cell 1978;15:261
Additional Bibliography
(追加文献)
Not specified
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No