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細胞:MCF-7

Data

Catalogue No.
(カタログ番号)
86012803
(ご注文時カタログ番号 : 【凍結】EC86012803-F0 【培養状態】EC86012803-G0)
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=MCF7+
Cell Line Name
(細胞名)
MCF7
Keywords
(キーワード)
Human Caucasian breast adenocarcinoma
Cell Line Description
(細胞概要)
Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Species
(種)
Human
Tissue
(組織)
breast
Morphology
(形態)
Epithelial-like
Growth Mode
(増殖様式)
Adherent
DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium
(適応培地)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Karyotype
(核型)
2n = 46, hypertriploid to hypotetraploid
Receptors Reported to express the oestrogen and progesterone receptor
Depositor
(供託者)
Dr M Shaw, ICI Pharmaceuticals Ltd
Originator
(創作者)
No
Country
(国名)
UK
References
(文献)
J Nat Cancer Inst 1973;51:1409; Cancer Res 1993;53:5882
Additional Bibliography
(追加文献)
Abaan OD, Polley EC, Davis SR, Zhu YJ, Bilke S, Walker RL, Pineda M, Gindin Y, Jiang Y, Reinhold WC, Holbeck SL, Simon RM, Doroshow JH, Pommier Y, Meltzer PS.2013 The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology. Cancer Res. 73(14):4372-82. PMID: 23856246.
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No

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