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細胞:MC3T3-E1

Data

Catalogue No.
(カタログ番号)
99072810
(ご注文時カタログ番号 : 【凍結】EC99072810-F0 【培養状態】EC99072810-G0)
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=99072810
Cell Line Name
(細胞名)
MC3T3-E1
Keywords
(キーワード)
Mouse C57BL/6 calvaria
Cell Line Description
(細胞概要)
The osteoblastic cell line MC3T3-E1 has been established from a C57BL/6 mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the resting state. Cells have the capacity to differentiate into osteoblasts and osteocytes and have been demonstrated to form calcified bone tissue in vitro. Mineral deposits have been identified as hydroxyapatite. Expression of basic fibroblast growth factor (bFGF) mRNA and protein has been shown to be regulated by treatment with TGF beta and bFGF. Prostaglandin F2a has been reported to stimulate DNA synthesis and proliferation by up-regulation of insulin-like growth factor I receptors. MC3T3-E1 secrete collagen and express murine leukemia inhibitory factor (mLIF) in RNA.
Species
(種)
Mouse
Tissue
(組織)
Calvaria
Morphology
(形態)
Fibroblast-like
Growth Mode
(増殖様式)
Adherent
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) seeding at 0.5 -2 x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA 5% CO2 37?C.Never allow the culture to become fully confluent
Culture Medium
(適応培地)
MEM alpha + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype
(核型)
Not specified
Products(産生物) Collagen
Depositor
(供託者)
Obtained from Riken Cell Bank, Japan
Originator
(創作者)
No
Country
(国名)
Japan
References
(文献)
J Biol Chem 1994 269 9392, Biochem Biophys Res Commun 1991 175:577, J Biol Chem 1991 266:21044, J C
Additional Bibliography
(追加文献)
Not specified
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No

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