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汎用セルライン(株細胞)17品目一覧

細胞:HepG2

Data

Catalogue No.
(カタログ番号)
85011430
(ご注文時カタログ番号 :  【培養状態】EC85011430-G0)
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=hepg2
Cell Line Name
(細胞名)
Hep G2
Keywords
(キーワード)
Human Caucasian hepatocyte carcinoma
Cell Line Description
(細胞概要)
The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g. albumin, alpha2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. The cells will respond to stimulation with human growth hormone.
Species
(種)
Human
Tissue
(臓器)
Liver
Morphology
(形態)
Epithelial
Growth Mode
(増殖様式)
Adherent
DNA Profile

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10
THO1: 9
TPOX: 8,9
vWA: 17
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells might take 6 days to form a confluent monolayer without media change if seeded at 1:4. Until then growth occurs mainly in islands.
Culture Medium
(適応培地)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Karyotype
(核型)
Modal no. 55
Products
(産生物)
Prothrombin, antithromin III, alpha-foetoprotein complement, C3 and C4 activator, fibrinogen
Depositor
(供託者)
Prof B Knowles, Wistar Institute, Philadelphia
Originator
(創作者)
No
Country
(国名)
USA
References
(文献)
Nature 1979;282:615; Science 1980;209:497; In Vitro Cell Dev Biol 1987;23:349
Additional Bibliography
(追加文献)
Not specified
Patents
(特許)
This material is cited in a US and/or other Patent and may not be used to infringe parent claims.
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No