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細胞:293(HEK293)

Data

Catalogue No.
(カタログ番号)
85120602
(ご注文時カタログ番号 【培養状態】EC85120602-G0)

■製品詳細アドレス:http://www.saibou.jp/service/kensaku/detail.php?catalogno=EC85120602-G0

Cell Line Name
(細胞名)
2931
Also Known As(別名) HEK 293
Keywords
(キーワード)
Human Embryo Kidney
Cell Line Description
(細胞概要)

Transformed with sheared human Ad5 DNA. Sensitive to human adenoviruses and adenovirus DNA. Can be used to isolate transformation defective host-range mutants of Ad5 and for titrating human adenoviruses. This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2%. The der (1)t(1;15) (q42;q13), der(19)t(3;19)(q12;q13),der(12)t(8;12) (q22;p13) and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+ and a single Xp+ in most cells. The Ad insert was shown to consist of a colinear segment from nucleotides 1 to 4344 integrated into chromosome 19 (19q13.2). Expression of an unusual cell surface receptor for vitronectin has been reported. This is composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.

PLEASE NOTE: 293 cells detach at room temperature; may take up to seven days to reattach.

GMO Status Genetically Modified Organism Class 1 (GMO1)
Species
(種)
Human
Tissue
(組織)
Kidney, embryo
Morphology
(形態)
Epithelial
Growth Mode
(増殖様式)
Adherent
DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. When resuscitating a frozen ampoule of cells we recommend the cells are seeded at the higher seeding level i.e. 5 x10,000/cm2. Cells may take up to 7 days to attach after resuscitation and subculture. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and reseed to allow re-attachment of cells. These cells should be frozen in 5% DMSO : 95% FCS
Culture Medium
(適応培地)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FCS
Karyotype
(核型)
2n 46, hypotriploid, modal no. 64
Depositor
(供託者)
Obtained from ATCC
Originator
(創作者)
No
Country
(国名)
USA
References
(文献)
Virology 1977 77:319 PNAS USA 1996 93:4891 PNAS USA 1996 93:4192 Virology 1978 86:10 J Biol Chem
Additional Bibliography
(追加文献)
(1) FEBS Lett 1996 Nov 11: 397(1): 39-44
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No