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細胞:CHO-K1

Data

Catalogue No.
(カタログ番号)
85051005(ご注文時カタログ番号
【凍結】EC85051005-F0:115,000円  【培養状態】EC85051005-G0):120,000円)
Cell Line Name
(細胞名)
CHO-K1
Keywords
(キーワード)
Hamster Chinese ovary
Cell Line Description
(細胞概要)

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

Species
(種)
Hamster
Tissue
(組織)
Ovary
Cell Type
(細胞タイプ)
Epithelial
Growth Mode
(細胞様式)
Adherent
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium
(適応培地)
Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype
(核型)
Hypodiploid, modal no.20
Depositor
(供託者)
Dr D Newell, PHLS CAMR, Porton Down, Salisbury
Originator
(創作者)
No
Country
(国名)
UK
References
(文献)
J Exp Med 1958;108:945Proc Nat Acad Sci USA 1968;60:1275
Additional Bibliography
(追加文献)
Not specified
Patents
(特許)
None specified by Depositor
Release Conditions
(供給条件)
No

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