細胞.jp
試薬事業部トップへ戻る
ケー・エー・シー
文字のサイズ
汎用セルライン(株細胞)17品目一覧

細胞:CACO2

Data

Catalogue No.
(カタログ番号)
86010202
(ご注文時カタログ番号 【培養状態】 EC86010202-G0)
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=CACO2
■トランズウエルプレートに播種した状態でのキット詳細アドレス:http://www.saibou.jp/service/poca/
Cell Line Name
(細胞名)
CACO-2
Keywords
(キーワード)
Human Caucasian colon adenocarcinoma
Cell Line Description
(細胞概要)
Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.
Species
(種)
Human
Tissue
(組織)
colon
Morphology
(形態)
Epithelial
Growth Mode
(増殖様式)
Adherent
DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11
D13S317: 11,13,14
D16S539: 12,13
D5S818: 12,13
D7S820: 11,12
THO1: 6
TPOX: 9,11
vWA: 16,18
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. NB: During routine subculture the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single cell suspension when split.
Culture Medium
(適応培地)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Karyotype
(核型)
Hypertetraploid, modal no. 96
Depositor
(供託者)
Dr J Clarke, AVRI, Pirbright
Originator
(創作者)
No
Country
(国名)
UK
References
(文献)
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Additional Bibliography
(追加文献)
Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832 Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No

Product Documents