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汎用セルライン(株細胞)17品目一覧

細胞:C2C12

Data

Catalogue No.
(カタログ番号)
91031101
(ご注文時カタログ番号 : 【凍結】EC91031101-F0 【培養状態】EC91031101-G0))
■製品詳細アドレス : http://www.saibou.jp/service/kensaku/result.php?keyword=91031101
Cell Line Name
(細胞名)
C2C12
Keywords
(キーワード)
Mouse C3H muscle myoblast
Cell Line Description
(細胞概要)
Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Species
(種)
Mouse
Tissue
(組織)
muscle
Morphology
(形態)
Myoblast
Growth Mode
(増殖様式)
Adherent
Subculture Routine
(継代方法/培養方法)
Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Do not allow cells to reach confluence.
Culture Medium
(適応培地)
DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS)
Karyotype
(核型)
Not specified
Depositor
(供託者)
Obtained from ATCC
Originator
(創作者)
No
Country
(国名)
USA
References
(文献)
Nature 1977;270:725, Science 1985;230;758-766, J. Cell Biol. 1994; 127:1755-1766[published erratum appears in J Cell Biol 1995 Feb;128(4):following 713], J.Virol. 1997;71:169-178, Proc. Natl. Acad.Sci. USA 1996;93:14082-14087, J. Biol. Chem. 1996;271:1386
Additional Bibliography
(追加文献)
(1)J Anim Sci; 1996 Jun: 74(6): 1265-73
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No

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