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細胞:A549

Data

Catalogue No.
(カタログ番号)
86012804
(ご注文時カタログ番号:【凍結】EC86012804-F0 【培養状態】EC86012804-G0))
■製品詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=EC86012804
Cell Line Name
(細胞名)
A549
Keywords
(キーワード)
Human Caucasian lung carcinoma
Cell Line Description
(細胞概要)
Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.
Species
(種)
Human
Tissue
(組織)
lung
Morphology
(形態)
Epithelial
Growth Mode
(増殖様式)
Adherent
DNA Profile

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium
(適応培地)
Ham's F12K or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype
(核型)
Hypotriploid
Products(産生物) Lecithin, High amounts of de-saturated fatty acids.
Depositor
(供託者)
Obtained from ATCC
Originator
(創作者)
No
Country
(国名)
USA
References
(文献)
Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP. 1973 In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.J Natl Cancer Inst. 51(5):1417-23. PMID: 4357758
Additional Bibliography
(追加文献)
Lieber M, Smith B, Szakal A, Nelson-Rees W, Todaro G 1976 A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer. 15;17(1):62-70. PMID: 175022. Abaan OD, Polley EC, Davis SR, Zhu YJ, Bilke S, Walker RL, Pineda M, Gindin Y, Jiang Y, Reinhold WC, Holbeck SL, Simon RM, Doroshow JH, Pommier Y, Meltzer PS.2013 The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology. Cancer Res. 73(14):4372-82. PMID: 23856246.
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No