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細胞:3T3-L1

Data

Catalogue No.
(カタログ番号)
86052701
(ご注文時カタログ番号: 【凍結】EC86052701-F0 【培養状態】EC86052701-G0)

詳細アドレス:http://www.saibou.jp/service/kensaku/result.php?keyword=86052701

Cell Line Name
(細胞名)
3T3 L1
Keywords
(キーワード)
Mouse Embryo
Cell Line Description
(細胞概要)

3T3 L1 is a continuous strain of 3T3 developed through clonal isolation. The cells are not contact inhibited. Cells can be induced to become adipose-like using the method described in the subculture routine information. Appearance of adipocytes can take weeks to achieve.

We would like to manage customer expectations with regard to the potential of the current 3T3 cell line stocks to differentiate into adipocytes. If you intend to use the cells for adipocyte differentiation please note: When cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g. 2 - 5 weeks, and the proportion of the population which differentiates can be limited. If you have previously used 3T3 cells from an alternative source we cannot guarantee the differentiation performance will be the same.<.p>

We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.

Species
(種)
Mouse
Tissue
(組織)
Embryo
Morphology
(形態)
Fibroblast-like
Growth Mode
(増殖様式)
Adherent
Subculture Routine
(継代方法/培養方法)
Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 2-4x10,000 cells/cm2 in a 75cm2 flask using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Never allow the culture to become fully confluent and subculture every 3 days. To stimulate differentiation into adipocytes grow the cells to confluency using the DMEM + 10% calf serum. 2 days after confluency induce differentiation by adding 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 0.25uM Dexamethoasone and 1ug/ml Insulin in DMEM with 10% Foetal Bovine Serum (FBS). After 2 days remove the IBMX and dexamethasone but maintain with insulin for another 2 days. On day 4, after inducing differentiation, and thereafter, culture the cells in DMEM with 10% FBS. Change medium every second day. Differentiation may take several weeks to occur e.g. 2 - 5 weeks. Differentiation begins in patches but with time a significant percentage of the population should change. Before any experiments on the differentiated cells incubate in serum-free DMEM for 2 hours.
Culture Medium
(適応培地)
DMEM + 2mM Glutamine + 10% Calf Serum (CS)
Karyotype
(核型)
2n = 40; Aneuploid with unstable karyotype
Depositor
(供託者)
Obtained from ATCC
Originator
(創作者)
No
Country
(国名)
USA
References
(文献)
Cell 1974;1:113;Cell 1974;3:127, J Biol Chem 1985;260:2646-2652
Additional Bibliography
(追加文献)
Evidence for the involvement vicinal sulfhydryl groups in insulin-activated hexose transport by 3t3-l1 adipocytes. By SC Frost and MD Lane
Patents
(特許)
None specified by Depositor
Research Council Deposit
(研究会への供託)
No
Release Conditions
(供給条件)
No

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